Helixyte Green 雙鏈DNA定量試劑

Helixyte Green dsDNA染料是美國AAT Bioquest生產(chǎn)的一種超靈敏熒光核酸染料，用于定量溶液中的雙鏈DNA（dsDNA）。近期，Helixyte Green dsDNA染料用于PCR產(chǎn)物的直接循環(huán)測序方法中的PCR擴(kuò)增產(chǎn)量。使用標(biāo)準(zhǔn)光譜熒光計(jì)和2.5 ng / mL帶有熒光酶標(biāo)儀的dsDNA檢測到少至25 pg / mL的dsDNA（在2 mL測定體積中50 pg dsDNA），在ssDNA，RNA和ss存在下檢測效果小 游離核苷酸。該測定在三個(gè)數(shù)量級(jí)上是線性的并且?guī)缀鯖]有序列依賴性。它是從多種來源準(zhǔn)確測量DNA的理想選擇，包括基因組DNA，病毒DNA，小量制備DNA或PCR。百螢生物是AAT Bioquest的中國代理商，為您提供的Helixyte Green 雙鏈DNA定量試劑

參考文獻(xiàn)

Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay
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Validation of a PicoGreen-based DNA quantification integrated in an RNA extraction method for two-dimensional and three-dimensional cell cultures
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Journal: Biophys J (2010): 3010

Comparison of SYBR Green I-, PicoGreen-, and [3H]-hypoxanthine-based assays for in vitro antimalarial screening of plants from Nigerian ethnomedicine
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Journal: Parasitol Res (2010): 933