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Ex (nm)
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-
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Em (nm)
|
-
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分子量
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~12000
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溶劑
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Water
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|
存儲條件
|
在零下15度以下保存, 避免光照
|
|
|
產品基本信息
產品名稱：RatioWorks Cal-520/ zFluor 647 -葡聚糖綴合物* MW 10,000 *
儲存條件：-15℃避光防潮
保質期：12個月
產品物理化學光譜特性
分子量：~12000
溶劑：水
鈣測量對于許多生物學研究至關重要。在結合鈣后顯示光譜響應的熒光探針使研究人員能夠通過使用熒光顯微鏡，流式細胞儀，熒光光譜和熒光酶標儀來研究細胞內游離鈣濃度的變化。可以使用patch pipette或顯微注射將細胞不滲透葡聚糖的鈣指示劑物理加載到細胞上。使用熒光顯微鏡檢測來自這些細胞的熒光信號。與AM酯形式相比，我們的鈣指示劑的右旋糖酐形式很少出現(xiàn)在外漏和區(qū)室化的問題。在熒光鈣指示劑右旋糖酐結合物中，Cal-520右旋糖酐結合物可能是好的選擇，因為它們的高熒光量子產率和鈣的大量熒光增強作用。 RatioWorks Cal-520®/ zFluor 647 -右旋糖酐綴合物帶有不依賴鈣的iFluor 647熒光探針作為放射測量的參考色。 Cal-520熒光團可以被488 nm的氬激光很好地激發(fā)，其中iFluor 647的激發(fā)少。參比iFluor 647熒光團可以被633 nm的He-Ne激光或647 nm的紅色激光很好地激發(fā)，其中Cal-520熒光團具有小的激發(fā)。與Cal-520L葡聚糖（＃20603）相比，此Cal-520葡聚糖對鈣離子具有更高的親和力，并且經(jīng)過優(yōu)化可監(jiān)測低水平的鈣離子。
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