鈣離子熒光探針Cal-520N, AM

鈣離子熒光探針Cal-520N, AM是美國(guó)AAT Bioquest生產(chǎn)的用于標(biāo)記鈣離子的熒光探針，Cal-520®提供強(qiáng)大的均勻熒光測(cè)定工具，用于檢測(cè)細(xì)胞內(nèi)鈣動(dòng)員。Cal-520®AM是一種新型熒光鈣敏感染料，與現(xiàn)有的綠色鈣指示劑（如Fluo-3 AM和Fluo-4 AM）相比，具有顯著信噪比和細(xì)胞內(nèi)保留。表達(dá)通過(guò)鈣信號(hào)傳導(dǎo)的GPCR或鈣通道的細(xì)胞可以預(yù)裝載可以穿過(guò)細(xì)胞膜的Cal-520 AM。一旦進(jìn)入細(xì)胞內(nèi)，Cal-520 AM的親脂性阻斷基團(tuán)被酯酶裂解，產(chǎn)生帶負(fù)電荷的熒光染料，留在細(xì)胞內(nèi)。與鈣結(jié)合后，其熒光大大增強(qiáng)。當(dāng)用激動(dòng)劑刺激細(xì)胞時(shí)，該受體表示細(xì)胞內(nèi)鈣的釋放，這顯著增加了Cal-520®的熒光。Cal-520®AM具有長(zhǎng)波長(zhǎng)，高靈敏度和> 100倍熒光增強(qiáng)的特性，是測(cè)量細(xì)胞鈣的理想指示劑。高信噪比和更好的細(xì)胞內(nèi)保留使Cal-520®鈣測(cè)定成為評(píng)估GPCR和鈣通道靶標(biāo)以及篩選其激動(dòng)劑和拮抗劑的有力工具。百螢生物是AAT Bioquest 的中國(guó)代理商，為您提供的鈣離子熒光探針

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